Rapid apolipoprotein E phenotyping by immunofixation in agarose
نویسندگان
چکیده
منابع مشابه
Rapid apolipoprotein E phenotyping by immunofixation in agarose.
Conventional determination of apolipoprotein E isomorphs comprises ultracentrifugation of 1-5 ml serum, delipidation of very low density lipoproteins (VLDL), and isoelectric focusing (IEF) in polyacrylamide gels. In order to reduce the sample volume and to avoid nonspecific protein bands, immunoblotting was proposed. Now we describe a methodological variant that uses 25 microliters serum, repla...
متن کاملimmunofixation on Thin-Layer Agarose
Na2CO3. After 1 mm, 1.0mL of alkaline picrate reagent (500 mL saturated picric acid, 100 mL of 1 mol/L NaOH, and 1 mL of Triton X-100 per liter) is added. The absorbance change at 505 nm and 37 #{176}C is measured between 48 s and 7 mm after adding the picrate reagent. Under these conditions, samples of pooled sera from jaundiced patients showed negative interference, 35 mg of total bilirubin p...
متن کاملApolipoprotein E genotyping on agarose gels.
References 1. Young DS, Bermes EW Jr. Specimen collection and processing-sources of biological variation. In: Burtis CA, Ashwood ER, eds. Tietz textbook of clinical chemistry, 2nd ed. Philadelphia: WB Saunders, 1994:58-101. 2. Lahiri DK, Schnabeh B. DNA isolation by a rapid method from human blood samples: effects of MgC12, EDTA, storage time, and temperature on DNA yield and quality. Biochem G...
متن کاملImmunofixation. I. General principles and application to agarose gel electrophoresis.
Immunofixation offers the worker an economical means of physically locating a protein in an electrophoretic strip and is ideally suited to forensic medicine, genetic studies, or research. The method is as simple and economical as the commonly used one- or two-dimensional immunoelectrophoresis, yet yields considerably more information.
متن کاملImproved phenotyping of apolipoprotein E: application to population frequency distribution.
A simple procedure for phenotyping apolipoprotein (apo) E directly from plasma has been developed for use in the lipid clinic laboratory. In this new method, 10 microL of serum or plasma is pretreated with neuraminidase (EC 3.2.1.18), which removes the sialic acid residues from apo E and eliminates additional bands, thereby ensuring correct phenotype assignment. After a rapid delipidation step,...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1991
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)42040-1